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zymo spin column  (Zymo Research)


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    Structured Review

    Zymo Research zymo spin column
    Zymo Spin Column, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zymo+spin+column/Zymo-Spin+I+Columns/pmc13116153-286-47-47
    Average 96 stars, based on 1086 article reviews
    zymo spin column - by Bioz Stars, 2026-09
    96/100 stars

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    Isolation:

    Article Title: Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systems
    Article Snippet: The RNA was eluted in 34 μL RNase-free water and DNA was removed by Turbo DNaseI treatment (Thermo Fisher Scientific, AM2239) (2 μL/sample) and incubated for 30 min at 37°C. .. After DNA degradation, the RNA isolation procedure using the Zymo Spin Column I was repeated and final RNA concentration was quantified by Nanodrop measurement. ..

    Concentration Assay:

    Article Title: Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systems
    Article Snippet: The RNA was eluted in 34 μL RNase-free water and DNA was removed by Turbo DNaseI treatment (Thermo Fisher Scientific, AM2239) (2 μL/sample) and incubated for 30 min at 37°C. .. After DNA degradation, the RNA isolation procedure using the Zymo Spin Column I was repeated and final RNA concentration was quantified by Nanodrop measurement. ..

    Purification:

    Article Title: Trait-specific chromatin architectures channel pleiotropic genes toward sexually dimorphic development in horned beetles
    Article Snippet: Once all samples were collected, RNA extractions were performed using a Direct-zol RNA Miniprep kit (Zymo Research). .. Briefly, tissues were thawed to 4 °C, homogenized in the Trizol with RNase-free polypropylene pestles, and purified following the protocol on a Zymo spin column with a DNA digestion treatment. .. Total RNA quantity and quality was measured using an Agilent 2200 TapeStation system with an RNA ScreenTape Assay.

    Article Title: Metagenomic and transcriptomic signatures of periodontitis in companion dogs
    Article Snippet: For RNA isolation, frozen host tissues (∼1 gm) were homogenized in 1 mL of Trizol (Thermo Fisher) using 2.8 mm ceramic beads (Hard Tissue Homogenizing Mix, VWR). .. RNA extraction was performed with a modified Trizol method as follows: after the addition of chloroform and phase separation of the Trizol lysate, the aqueous phase was combined with an equal volume of 100% ethanol and loaded onto a Zymo-Spin column and purified using the Quick-RNA Prep Kit (Zymo Research). .. For all samples, RNA concentration was measured with a Nanodrop (Thermo Fisher), and integrity was determined with a Fragment Analyzer (Agilent).

    Article Title: AID and TET2 cooperate to demethylate Irf4 for plasma cell fate in germinal center B cells
    Article Snippet: Inducible GC B cells from day 7 were subjected to CpG methylation analysis using EZ DNA Methylation-Direct Kit (Cat. #D5021; Zymo Research). .. In brief, 1 × 10 4 iGB cells were lysed for 20 min at 50° with M-digestion buffer containing proteinase K. Cell lysates containing DNA were directly subjected to bisulfite conversion for 3.5 h. Reaction was stopped using desulfonation buffer, and then, DNA was purified with a Zymo-Spin column. ..

    RNA Extraction:

    Article Title: Metagenomic and transcriptomic signatures of periodontitis in companion dogs
    Article Snippet: For RNA isolation, frozen host tissues (∼1 gm) were homogenized in 1 mL of Trizol (Thermo Fisher) using 2.8 mm ceramic beads (Hard Tissue Homogenizing Mix, VWR). .. RNA extraction was performed with a modified Trizol method as follows: after the addition of chloroform and phase separation of the Trizol lysate, the aqueous phase was combined with an equal volume of 100% ethanol and loaded onto a Zymo-Spin column and purified using the Quick-RNA Prep Kit (Zymo Research). .. For all samples, RNA concentration was measured with a Nanodrop (Thermo Fisher), and integrity was determined with a Fragment Analyzer (Agilent).

    Modification:

    Article Title: Metagenomic and transcriptomic signatures of periodontitis in companion dogs
    Article Snippet: For RNA isolation, frozen host tissues (∼1 gm) were homogenized in 1 mL of Trizol (Thermo Fisher) using 2.8 mm ceramic beads (Hard Tissue Homogenizing Mix, VWR). .. RNA extraction was performed with a modified Trizol method as follows: after the addition of chloroform and phase separation of the Trizol lysate, the aqueous phase was combined with an equal volume of 100% ethanol and loaded onto a Zymo-Spin column and purified using the Quick-RNA Prep Kit (Zymo Research). .. For all samples, RNA concentration was measured with a Nanodrop (Thermo Fisher), and integrity was determined with a Fragment Analyzer (Agilent).

    Incubation:

    Article Title: Development of a rapid and modified total RNA extraction method from polyphenolic-rich Gossypium hirsutum
    Article Snippet: .. Add an equal volume of ice-cold 100% isopropanol, mix gently and load on zymo-spin column of DirectZol kit (original method includes isopropanol addition, incubation at -20 °C for 30 min and then centrifugation at 14000 rpm at 4 °C). b. .. Add equal volume of 5M ammonium acetate, mix thoroughly and load the content on zymo-spin column of DirectZol kit. c. Add equal volume of 5M ammonium acetate, mix gently and keep on ice for 30 min, centrifuge at 14000 rpm at 4 °C for 20 min and keep pellet.

    Centrifugation:

    Article Title: Development of a rapid and modified total RNA extraction method from polyphenolic-rich Gossypium hirsutum
    Article Snippet: .. Add an equal volume of ice-cold 100% isopropanol, mix gently and load on zymo-spin column of DirectZol kit (original method includes isopropanol addition, incubation at -20 °C for 30 min and then centrifugation at 14000 rpm at 4 °C). b. .. Add equal volume of 5M ammonium acetate, mix thoroughly and load the content on zymo-spin column of DirectZol kit. c. Add equal volume of 5M ammonium acetate, mix gently and keep on ice for 30 min, centrifuge at 14000 rpm at 4 °C for 20 min and keep pellet.



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